Bacterial Microbiota of Canine Corneal Ulcers Treated with Tilapia Skin Graft: Implications for Postoperative Ocular Safety
Abstract:
Corneal ulcers in dogs are frequently associated with microbial contamination, which may compromise healing and lead to severe ocular complications. The use of tilapia skin as a biotechnological graft has emerged as a promising alternative for corneal repair; however, its influence on the ocular microbiota remains poorly understood. This study aimed to evaluate the bacterial microbiota of canine corneal ulcers treated with tilapia skin graft, focusing on the presence and evolution of microorganisms during the surgical and postoperative periods. Microbiological samples were collected from dogs presenting deep corneal ulcers or perforations, using sterile swabs applied to both central and peripheral regions of the lesion. Samples were transferred to Stuart transport medium and subjected to standard microbiological analysis. Initial cultures revealed the presence of clinically relevant bacteria, including Pseudomonas spp. and Klebsiella spp., commonly associated with ocular infections. Postoperative evaluation demonstrated a notable reduction in bacterial growth, with no detectable microbial proliferation after 20 days of treatment under topical antibiotic therapy. These findings suggest that the use of tilapia skin graft does not promote microbial persistence and may be associated with a favorable microbiological environment during the healing process. This study highlights the importance of monitoring ocular microbiota in corneal repair procedures and supports the safety of tilapia skin as a biotechnological material in veterinary ophthalmology. Further studies with larger sample sizes are required to confirm these findings and explore long-term microbiological dynamics.
KeyWords:
Corneal ulcer; canine; ocular microbiota; tilapia skin graft; xenograft; veterinary ophthalmology
References:
- Costa BA, Lima Júnior EM, Moraes Filho MO, et al. Use of tilapia skin as a biological graft in tissue repair: a review. Revista Brasileira de Cirurgia Plástica. 2021;36(2):220–227. https://doi.org/10.5935/2177-1235.2021RBCP0025
- Gelatt KN, Gilger BC, Kern TJ. Veterinary Ophthalmology. 6th ed. Wiley-Blackwell; 2021.
- Hindley KE, Groth AD, King M, Graham K, Billson FM. Bacterial isolates, antimicrobial susceptibility, and clinical characteristics of bacterial keratitis in dogs presenting to a referral practice in Australia. Veterinary Ophthalmology. 2016;19(5):418–426. https://doi.org/10.1111/vop.12325
- Markey BK, Leonard FC, Archambault M, et al. Clinical Veterinary Microbiology. 2nd ed. Elsevier; 2013.
- Lima Júnior EM, et al. Innovative treatment using tilapia skin as a xenograft for partial thickness burns after a gunpowder explosion. Journal of Surgical Case Reports. 2019;2019(6):rjz181. https://doi.org/10.1093/jscr/rjz181
- Melo MS, et al. Tilapia skin graft (Oreochromis niloticus) in the repair of canine corneal ulcer: case report. Brazilian Journal of Animal and Environmental Research. 2022;5:367–375.
- Melo MS, et al. Use of occlusive tilapia skin dressing in corneal perforation with lens luxation and extrusion in a puppy: case report. Revista Científica Veterinária. 2023. https://doi.org/10.56083/RCV3N11-015
- Melo MS, et al. Tilapia skin as a graft in the repair of corneal ulcers using biotechnological derivatives in dogs. Ciência Animal. 2024;34(2):152–158. https://revistas.uece.br/index.php/cienciaanimal/article/view/13475
- Melo MS, et al. Use of tilapia skin scaffold (Oreochromis niloticus) in corneal ulcer repair in Amazona aestiva: case report. PubVet. 2025;19(6):e1792. https://doi.org/10.31533/pubvet.v19n06e1792
- Quinn PJ, Markey BK, Leonard FC, et al. Veterinary Microbiology and Microbial Disease. 2nd ed. Wiley-Blackwell; 2011.
- Tsvetanova A, et al. Melting corneal ulcers (keratomalacia) in dogs: A 5-year clinical and microbiological study (2014–2018). Veterinary Ophthalmology. 2021;24(3):265–278. https://doi.org/10.1111/vop.12885